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If an Invitrogen™ antibody isn't going to perform as described on our Web site or datasheet,we are going to swap the solution for gratis for you, or offer you a credit history for your long run buy.*

The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has actually been noticed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was uncovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but detailed nucleotide sequence facts just isn't nonetheless offered.

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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide typically has the epitope acknowledged because of the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...

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This tutorial outlines further more possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...

Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.

Goal Details Cyclin E belongs for the very conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E varieties a posh with and functions being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells development via S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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